Genepharma fish
WebOct 26, 2016 · FISH assay. FISH assay was carried out according to previous studies. 27, 28 Briefly, cells were rinsed briefly in PBS and then fixed in 4% formaldehyde for 10 min at room temperature. Cells were ... WebAug 25, 2024 · Phosphatidylinositol transfer protein membrane-associated 1 (PITPNM1) contains a highly conserved phosphatidylinositol transfer domain which is involved in phosphoinositide trafficking and signaling transduction under physiological conditions. However, the functional role of PITPNM1 in cancer progression remains unknown. Here, …
Genepharma fish
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http://www.genepharma.com/products.php WebFish assay. Prepare the probes. Then, a larger wet box was used to place the slices crosswise, and 10-µL probes were dropped on the sliced tissue. Add a coverslip, cover the wet box and incubate at 37°C for 12-16 h.
WebNational Center for Biotechnology Information WebARTICLE OPEN Novel roles of LSECtin in gastric cancer cell adhesion, migration, invasion, and lymphatic metastasis Yinan Zhang1,2, Zhen Feng 1, Yue Xu 2, Sufen Jiang , Qianshi Zhang , Zhenyu ...
WebMay 25, 2024 · FISH was conducted (GenePharma), and probe signal on cells was observed under fluorescent microscopy (Carl Zeiss, Jena, Germany). Tumor formation assay. Female nude 4–5-week-old mice were used for the assay. After transfection of circ-PTK2 plasmids or control plasmids for 48 h, SK-OV-3 cells (5 ... WebMay 12, 2024 · These probes were all synthesized by GenePharma. FISH staining was performed according to the manufacturer’s instructions (GenePharma). DAPI was used to stain the cell nuclei. A confocal laser scanning microscope (TCS SP8; Leica, Wetzlar, Germany) was used to observe the subcellular distribution of circRUNX1 and miR-145 …
WebApr 8, 2024 · After permeabilization with 0.25% Triton X-100 for 10 min, the cells were washed with PBS and hybridised with FISH probes (designed by RiboBio) overnight in a humidified chamber at 37 °C in the dark.
WebNov 1, 2024 · The Cy3-labeled lncRNA Gm26917 probe was designed and synthesized by GenePharma (Shanghai, China). ... FISH was conducted according to the manufacturer’s instructions (GenePharma). Cells grown on Collagen I 22-mm round coverslips (354089; Corning) were fixed in 4% paraformaldehyde for 15 min and treated with 0.1% Triton X … fnb hummelstown paWebThe FISH experiment was carried out according to the instruction of the RNA FISH kit (GenePharma) and images were photographed by confocal microscopy (Leica Microsystems). All probe sequences used for the FISH assay are summarized in Additional file 1 Table S1. CircRNA pull-down assay. fnb home loan additional amountWebGenepharm is focused on the development, manufacturing and marketing of generic pharmaceuticals. Use the CB Insights Platform to explore Genepharm's full profile. fnbhs.com sign inWebNational Center for Biotechnology Information fnbhs online bankingWebThe experiment was performed in 4 groups, including cells transfected with NC (negative control of miR‐2834 mimics, GenePharma, Shanghai, China) and pIEX4‐EGFP, ... Subcellular localization of lncR26319 by FISH showed that lncR26319 was present in both the nucleus and cytoplasm, ... fnb huntingdon pa hoursWebRNA FISH常规试剂盒 ... [email protected]. [email protected]. 传真：0512-86665900. 关于我们 - 吉玛基因 - GenePharma 动物实验服务 - 吉玛基因 - GenePharma 信息核对 - 吉玛基因 - GenePharma 吉玛基因由入选国家重大人才引进工程的张佩琢博士领衔的海归创业团队，于2003 … 技术支持 - 吉玛基因 - GenePharma 订购商城 - 吉玛基因 - GenePharma siRNA产品 - 吉玛基因 - GenePharma shRNA产品 - 吉玛基因 - GenePharma miRNA产品 - 吉玛基因 - GenePharma green tea with fruitWebNov 18, 2024 · FISH was performed according to the manufacturer’s instruction (GenePharma, Shanghai, China). Translation analysis After 48 h of transfection, treating cells with fresh DMEM containing 10 µg/mL puromycin for 5 min at 37 °C before harvesting. green tea with ginger and honey benefits