Maltose binding protein uniprot

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August undefined, 2024

WebJun 3, 2011 · Using x-ray crystallography, we have captured the maltose transporter in an intermediate step between the inward- and outward-facing states. We show that interactions with substrate-loaded maltose-binding protein in the periplasm induce a partial closure of the MalK dimer in the cytoplasm. ATP binding to this conformation then promotes ... WebSep 1, 2024 · Uniprot ID P37288 Uniprot Name Vasopressin V1a receptor Molecular Weight ... A C-terminal segment of the V1R vasopressin receptor is unstructured in the crystal structure of its chimera with the maltose-binding protein. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt 4):341-5. Epub 2005 Mar 24.

CELL CULTURE MEDIUM FOR EUKARYOTIC CELLS

WebMaltose binding protein (MBP) is used in recombinant protein expression as an affinity and solubility tag. The monoclonal antibody B48 binds MBP tightly and has no cross-reactivity to other proteins in an Escherichia coli lysate. WebFeb 8, 2024 · The Uniprot database predicted that DogH was ... and trehalose was decreased by 2.6 times to 7.2 μg mg protein −1. The maltose and trehalose content in the WT-dogH-over overexpression strain were ... A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye … good twitch names not taken

Maltose-binding protein - Wikipedia

WebAug 31, 2024 · ALG-2 is a penta-EF-hand Ca2+-binding protein and interacts with a variety of proteins in mammalian cells. In order to find new ALG-2-binding partners, we searched a human protein database and retrieved sequences containing the previously identified ALG-2-binding motif type 2 (ABM-2). After selecting 12 high-scored sequences, we … WebAnti-Maltose Binding Protein Antibody, clone 1H4 ascites fluid, clone 1H4, Chemicon®; Synonyms: MBP; find Sigma-Aldrich-MAB3108 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich ... UniProt accession no. P68187. shipped in. dry ice. Gene Information. Escherichia coli K12 ... Male(948538) … WebWe have overexpressed 3CLpro in E. coli as a fusion protein with maltose binding protein (MBP). The MBP-3CLpro fusion protein was purified from contaminating E. coli proteins by amylose column chromatography, and r3CLpro was … chevy canyon mpg

Maltose-Binding Protein (MBP), a Secretion-Enhancing …

WebNov 17, 2015 · Searching Start2Fold can be performed by typing in protein names, UniProt/PDB IDs, experiment types, experiment methods and protection levels in the search field, and pressing ‘search’. ... Mapping the conformational stability of maltose binding protein at the residue scale using nuclear magnetic resonance hydrogen exchange … good twitch profile picturesWebMar 21, 2024 · MBL3P (Mannose-Binding Lectin Family Member 3, Pseudogene) is a Pseudogene. Additional gene information for MBL3P Gene HGNC (2221) NCBI Entrez Gene (50639) Ensembl (ENSG00000219430) Alliance of Genome Resources Search for MBL3P at DataMed Search for MBL3P at HumanCyc chevy canyon 4x4

"WebMaltose Binding Protein. MBP-intein-CBD is a model fusion protein containing three different protein domains: MBP as the affinity target, CBD as the protein of interest and … " - Maltose binding protein uniprot

Maltose binding protein uniprot

WebMaltose-Binding Protein. MBP-intein-CBD is a model fusion protein containing three different protein domains: MBP as the affinity target, CBD as the protein of interest and … WebThe insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP …

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WebMay 5, 2024 · In this study, we show that maltose-binding protein (MBP) is capable of facilitating stable gold nanoparticle synthesis, and a structure of MBP in the presence of … Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kilodaltons.

WebNew cell culture methods that provide even incremental improvements in recombinant protein production are valuable, given the challenges and expense of large scale cell culture processes and the growing demand for greater quantities of and lower costs for biological products. Improvements to cell culture processes, recombinant polypeptide ... Websemi-batch and fast protein liquid chromatography (FPLC) modes, and a new matrix-assisted dialysis refolding method is described that is suitable for batch and semi-batch modes. MBP, also referred to as maltodextrin-binding protein (and sometimes written unhyphenated), can be expressed at arguably the highest levels for any recombinant …

WebMar 30, 2016 · Here, we investigated expression levels of recombinant extracellular, intracellular as well as transmembrane proteins tethered to different polypeptides in … WebApr 21, 2011 · Author Summary Maltose-binding protein (MBP) is a bacterial protein involved in nutrient uptake. Crystallographic studies have revealed two stable …

WebTEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ S; however, the amino acid in the P1’ position can also be G, A, M, C, or H (1). It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from ...

WebThe maltose operon is an example of a positive control of transcription. When maltose is not present in E. coli, no transcription of the maltose genes will occur, and there is no maltose to bind to the maltose activator protein. This prevents the activator protein from binding to the activator binding site on the gene, which in turn prevents RNA … chevy cape codWeb3 Page Mutant Maltose binding protein (MBP) Applications Affinity purification, Detection, Improves solubility Comments Affinity purified using amylose resin. good twitch profile bannersWebMaltose-Binding Protein MBP-intein-CBD is a model fusion protein containing three different protein domains: MBP as the affinity target, CBD as the protein of interest and the intein domain inserted between MBP and CBD in view of its self-cleavage activity. From: Membrane Science and Technology, 2003 Add to Mendeley About this page chevy canyon truckWebMaltose binding protein (MBP) is used in recombinant protein expression as an affinity and solubility tag. The monoclonal antibody B48 binds MBP tightly and has no cross … good twitch rulesWebMaltose/maltodextrin-binding periplasmic protein Gene malE Status UniProtKB reviewed (Swiss-Prot) Organism Escherichia coli (strain K12) Amino acids 396 Protein existence Evidence at protein level Annotation score 5/5 Entry Feature viewer Publications … good twitch profile pictureWebJul 25, 1995 · 1MPD MALTODEXTRIN-BINDING PROTEIN (MALTOSE-BINDING PROTEIN) MUTANT, WITH ARGININE REPLACING TRYPTOPHAN AT POSITION 230 … good twitch streamer namesWebThe method is based on the availability of a target-binding nanobody, which is then rigidly attached to two scaffolds: (1) a Fab-fragment of an antibody directed against the nanobody, and (2) a nanobody-binding protein A fragment fused to maltose binding protein and Fab-binding domains. chevy canyon specs